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About hc-15

Information about the hc-15 data set.


The data presented here are raw extracellular voltage recordings of spontaneous action potentials from cultured mouse hippocampal neurons, recorded with multi-electrode arrays. Each recording displays several examples of action potential propagation in single neurons. These are seen as near-coincident spikes among groups of co-active electrodes, which occur in stereotyped sequences, with typically sub-millisecond inter-electrode latencies. We refer to the entirety of these multi-electrode events as propagation signals. Propagation signals are useful because they confirm that spikes at constituent electrodes all arise from single identifiable neurons and thus are a unique source of ground truth in the extracellular record; spikes from single neurons can be isolated from all other detected spikes in the extracellular record, independently of spike sorting routines.

We used MEA Viewer software to visualize the two dimensional extent and inter-electrode latencies of individual propagation signals. MEA Viewer is visualization software for multi-channel electrophysiological data, with features specifically designed to visualize propagation signals, taking advantage of the ability to signal average these events. Details of the features embedded in MEA Viewer, including the ability to visualize propagation signals, can be found here: 

MEA Viewer: A high-performance interactive application for visualizing electrophysiological data. Daniel C. Bridges, Kenneth R. Tovar, Bian Wu, Paul K. Hansma & Kenneth S. Kosik. DOI:

Download and installation details for MEA Viewer can be found here:

Results from our experiments, including experimental validation and methodological details, can be found here: 

Action potential propagation recorded from single axonal arbors using multi-electrode arrays. Kenneth R. Tovar, Daniel C. Bridges, Bian Wu, Connor Randall, Morgane Audouard, Jiwon Jang, Paul K. Hansma & Kenneth S. Kosik. Journal of Neurophysiology (2018)

Format of the data

The data are stored in hdf5 files. Details are given in the data description document listed at the bottom of this page.  Total data size is about 15 GB.

Conditions for using the data

If any work is published using the data, please cite the publication(s) above (Tovar et al., 2018 and/or Bridges et al., 2017) and please also cite this data set in the following:

Kenneth R. Tovar, Daniel C. Bridges, Bian Wu, Paul K. Hansma and Kenneth S. Kosik (2018); Extracellular voltage recordings of spontaneous action potentials from cultured mouse hippocampal neurons showing action potential propagation in single neurons.

The above citation uses a Digital Object Identifier (DOI) which is assigned to the data set.  The DOI was created using DataCite ( and the California Digital Library, "EZID" system (

How to download the data

The data may be downloaded from:
Using a account.  See the download link for more information about downloading.

Getting help using the data.

If you have questions about using the data, post them on the forum for using data sets.

Documentation files


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